hydrogen deuterium exchange mass spectrometry

Started by Melissa George · 0 Replies
Posted: 2 yrs
Hydrogen deuterium exchange mass spectrometry (HDX-MS) is a powerful and important tool used to study protein conformation and dynamics, protein-protein interactions, protein-small molecule interactions, and protein-RNA interactions. HDX-MS takes advantage of the naturally proton exchange occurring at amides on proteins, and deuterium can replace the protein backbone hydrogens upon exposure of the protein to a D2O-based buffer. According to the protein's structure, the exchange rates varies. If the hydrogen-bonded segments are tight, the exchange rate is low; the exchange rate is high when the regions are disordered. hydrogen deuterium exchange mass spectrometry
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